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李萌
2015-07-07 09:13     (点击: )

博士2003.9 –2006.7

第四军医大学生物化学与分子生物学专业

硕士2000.9 –2003.7

陕西师范大学生命科学学院细胞生物学专业

学士1996.9 –2000.7

陕西师范大学生命科学学院生物学专业

科研经历

l副教授2010.07-至今

第四军医大学药物基因组学教研室

科研方向:多肽及蛋白质类药物

l博士后2008.03- 2010.03

美国密西根大学医学院内科学系

科研方向:Affinity fluorescence-labeledpeptides for the early detection of cancer in Barrett’s Esophagus

l讲师2006.07- 2008.02

第四军医大学药学系生物制药学教研室

科研方向: Identify membrane-Associated TGF-β1’s function in tumor immunocompromise and purificationof some cytokines

相关技术::

lMammalian cell culture (various tumor cells, HUVEC,CHO)

lProtein expression and purification

lIsoelectric focusing

lSorting cells through immunomagnetic beads

lImmunoprecipitation

lchromatography

承担教学:蛋白质研究技术(硕士研究生课程)

l博士研究生2003.9 – 2006.7

第四军医大学生物化学与分子生物专业

科研方向To develop a mimotope vaccination forepitope-specific induction of anti-CD20 antibodies (supported by Openingfoundation of State Key Laboratory of Cancer Biology)

相关技术:

lscreening random phage display peptide libraries

lDNA purification (Genomic DNA & plasmid)

lRNA purification

lPCR, RT-PCR

l2D gel electrophoresis

lDNA sequence analysis(Internet and computer programs)

lExpression of recombinant protein in E.coli or yeast

lPurification of protein from Sf9 or E.coli usingchromatography

l(ion-exchange, gel-filtration, hydrophobicity andaffinity)

lSDS-PAGE gel and 2Dgel

lWestern blot and Dot blot

lMTT assay

lELISA

lFlow cytometry

lConstruction of tumor-bearing models

lTumor treatment experiments

lMammalian cell culture (RajiDaudiJurkatHela)

l硕士研究生2000.09 – 2003.07

陕西师范大学生命科学学院细胞生物学

科研方向:The killing effect of Ultrasound and Hematoporphyrinderivatives on Ehrlich ascites tumor (EAT) in vivo (supported by NationalNatural Science foundation of China, No.39870240; No.30270383)

相关技术:

lImmunohistochemistry, Immunocytochemistry andImmunofluorescence

lConstruction of tumor-bearing models

lTumor treatment experiments

lPharmacokinetics experiments

lBio-distribution experiments

lSingle-dose, Repeated-dose and genetic toxicityexperiments

科研基金

l国家自然科学基金面上项目,81272517、新型食管腺癌特异性结合多肽的导向功能研究及其靶分子鉴定、2013/01-2016/12、70万、在研、主持。

l重大新药创制科技重大专项军队特需药品保密专项,2011ZXJ09104-01B新型促战伤伤口愈合的基因工程药物-Tβ4②的临床前研究、2011/01-2013/12、270万、结题、主持。

l国家自然科学基金青年基金,30801002、Foxp3第二外显子缺陷突变体(Foxp3△2)相互作用蛋白的筛选与鉴定、2009/01-2011/12、19万、结题、主持。

l重组人干扰素与肿瘤新生血管结合多肽NGR融合蛋白的临床前研究(2009ZX09102-225)国家科技重大专项(第三名)2009年 500万

lNotch信号负调节因子在牙根发育启动中的作用研究;国家自然科学基金(30901675)国家自然科学基金青年基金(第二名)2009年20万

l利用广谱泛素连接酶降解致癌性受体酪氨酸激酶―一种联合干预多靶点的肿瘤治疗新策略(30873003)国家自然科学基金面上项目(第四名)2008年30万

lRNA结合蛋白QKI抑制COX2在胃癌发生发展中的作用(30900537)国家自然科学基金青年基金(第五名)2009年20万

lFoxp3通过UXT负向调控NF-κB的转录活性及其复合物在调节性T细胞中的作用(30972672)国家自然科学基金面上项目(第六名)2009年35万

国家发明专利

l人胸腺素β4二串体蛋白制备方法(中国发明专利)ZL201110376361.2(第三名)

lTARGETED DETECTION OF DYSPLASIA IN BARRETTs ESOPHAGUSWITH A NOVEL POLYPEPTIDE(国际发明专利)No.2010/0310459 A1(共同第一)

l人B细胞特异性膜分子CD20的12氨基酸模拟表位及其该表位构建的肿瘤疫苗(第二名)ZL200510096370.0

发表论文

(1)Ma X,Yuan Y, Zhang Z, Zhang Y,Li M. An analog of Ac-SDKP improves heartfunctions after myocardial infarction by suppressing alternative activation(M2) of macrophages. Int J Cardiol. 2014 Aug 1;175(2):376-8.IF 6.17通信作者

(2)Xu TJ,Wang Q, Ma XW, Zhang Z, Zhang W, Xue XC, Zhang C, Hao Q, Li WN, Zhang YQ,LiM. A novel dimeric thymosin beta 4 with enhanced activities accelerates therate of wound healing. Drug Des Devel Ther. 2013 Oct 1;7:1075-88.IF 3.48通信作者

(3)MengLi,Tianjiao Xu, Zhao Zhang, Xiaochang Xue, Cun Zhang, Xin Qin, Weina Li, QiangHao, Wei Zhang and Yingqi Zhang. A Phase II Multicenter, Randomized,Double-Blind study of a recombinant mutated human tumor necrosis factor-alphain combination with Chemotherapies in Cancer patients. Cancer Sci. 2012Feb;103(2):288-95.IF 3.4第一作者

(4)LiM,Anastassiades CP, Joshi B, Komarck CM, Piraka C, Elmunzer BJ, Turgeon DK,Johnson TD, Appelman H, Beer DG, Wang TD. Affinity peptide for detection ofdysplasia in Barrett's esophagus.Gastroenterology.2010 Nov;139(5):1472-80.IF 13.9第一作者

(5)LiM, Qin X,Xue X, Zhang C, Yan Z, Han W, Komarck C, Wang TD, Zhang Y. Safety evaluationand pharmacokinetics of a novel human tumor necrosis factor-alpha exhibited ahigher antitumor activity and a lower systemic toxicity. Anticancer Drugs. 2010Mar;21(3):243-51.第一作者

(6)LiM, WangTD. Targeted endoscopic imaging.Gastrointest Endosc Clin N Am. 2009 Apr;19(2):283-98.第一作者

(7)MengLi, Wei Han,Quiyang Zhang, Xiaochang Xue, Zenglu Wang and Yingqi Zhang.Development of a Mimotope-BasedVaccine Against CD20 Antigen. Protein Peptide Lett. 2007 Vol 14, No.6.610-614.第一作者

(8)MengLi, Zhen Yan,Wei Han, Yingqi Zhang. Mimotope vaccination for epitope-specific induction of anti-CD20antibodies. Cell Immunol. 2006 Feb;239(2):136-43第一作者

(9)Xin Q, CunZ, Xiaochang X,Meng L, Weina L, Qiang H, Yingqi Z, Zhen Y, Wei Z.Identification of a novel peptide ligand of human transfrrin receptor 1 fortargeted tumor delivery drug. Protein Pept Lett. 2012 Jun 5.

(10)Qin X, Wan Y,Li M,Xue X, Wu S, Zhang C, You Y, Wang W,Identification of anovel peptide ligand of human vascular endothelia growth factor receptor 3 fortargeted tumor diagnosis and therapy.J Biochem (Tokyo). 2007 May 21; [Epub ahead of print]

(11)Wan Y, Xue X,Li M, Zhang X, Qin X, Zhang C, You Y, Wang W, Jiang C, Wu S, Liu Y, Zhu W,Ran Y, Zhang Z, Han W, Zhang Y.Preparedand screened a modified TNF-alpha molecule as TNF-alpha autovaccine to treatLPS induced endotoxic shock and TNF-alpha induced cachexia in mouse.Cell Immunol. 2007 Jun 23; [Epub ahead ofprint]

(12)Meng Li, Quanhong Liu, Hao Qi, JinxuanZhang, Kun Zhang, Pan Wang. The scanning electron microscope observation of twokinds cancer cells treated by ultrasound irritating HpD. Journal of NorthwestUniversity. 2003 Apr.33(2):223-226.

(13)Meng Li, Yongmei Zhao, Pan Wang. APreliminary Report on the killing Effect of Ultrasound and Hematoporphyrin onEhrlick Ascites Tumor in Vivo. Journal of Xi’An United University. 2003Apr.6(2):9-12.

(14)Quanhong Liu, Pan Wang,MengLi, Hao Qi, Zhiyuan Shang, Yaohui Ren, Kun Zhang, Xiao Yao. Apopotosisof Ehrlich ascites tumor cells by sonochemical activated hematoporphyrin. ActaZoologica Sinica. 2003 49(5):620-628.

(15)Quanhong L, Shihui S, Yaping X, Hao Q,Jinxuan Z, Yaohui R,Meng Li, Pan W. Synergisticanti-tumor effect of ultrasound and hematoporphyrin on sarcoma180 cells,withspecial reference to the changes of morphology and cytochrome oxidaseactivity of tumor cells J.Exp.Clin.Cancer Res., 2004 23(2):333-341.

(16)Quanhong Liu, Shihui Sun,Yaping Xiao, Hao Qi, Zhiyuan Shang, Junping Zhang,Meng Li, QingLi. Study of cell killing and morphology on S180 by ultrasound activatinghematoporphyrin derivatives. Science In China (Series C).2003Jun.46(3):253-262.

(17)Kun Zhang, Hao Qi, QuanhongLiu, Qing Li,Meng Li. Effects of hematoporphyrin activated with lowerintensity focus ultrasound on SW2480 cells measured by MTT colorimetric assay. Journalof Shaanxi Normal University. 2003 31(1):93-98.

(18)冉永刚,李萌,韩苇,张英起,噬菌体随机12肽库中VEGF模拟表位的筛选及初步鉴定.第四军医大学学报2008, 29 (15)

(19)薛晓畅,李萌,秦鑫,李维娜,郝强,包春杰,韩苇,张英起,重组人B淋巴细胞刺激因子工程菌生物学特性的稳定性.中国生物制品学杂志. 2008, Vol. 21 No. 9

(20)陈霖,王伟华,张存,薛晓畅,李萌,李维娜,韩苇,颜真,张英起,重组人B72H1IgV疫苗对小鼠骨髓瘤细胞的抑制作用.第四军医大学学报(JFourthMilMed Univ) 2007, 28 (21)

(21)李萌,犹太民族的教育特点及其对我国教育发展的启示意义.唐都学刊。2009.Vol.10No.10

获得荣誉

第四军医大学“精英人才库”

第四军医大学“青年英才”

REFERENCES

lThomas D Wang, MD,PhD,

Divisionof Gastroenterology and Hepatology,

Universityof Michigan, School of Medicine,

109Zina Pitcher Place, BSRB 1522,

AnnArbor, MI., USA, 48109,

Fax:1-734-647-7950,

E-mail:thomaswa@umich.edu

lQuiyang (Daniel) Zhang, Ph.D.

ResearchInstructor,

Pulmonary& Critical Care Medicine

Universityof Texas Southwestern Medical Center

5323 Harry Hines Boulevard

Dallas, Texas 75390-9034

Tel: (214) 648-8623 (w), (972) 359-9977 (h),

E-mail:Daniel.Zhang@UTSouthwestern.edu

lYingqi Zhang,Ph.D.

Professor,Director

BiotechnologyCenter,

the Fourth Military Medical University

Changle Xilu Road#17, Xi’an, Shaanxi 710032, P. R. China

Tel: 86-29-84774775

e-mail:zhangyqh@fmmu.edu.cn

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